Summary information and primary citation
- PDB-id
- 8ifm; DSSR-derived features in text and JSON formats
- Class
- RNA binding protein-RNA-DNA
- Method
- cryo-EM (2.92 Å)
- Summary
- cryo-EM structure of tetrameric sparta grna-ssDNA target complex in state 2
- Reference
- Zhang JT, Wei XY, Cui N, Tian R, Jia N (2024): "Target ssDNA activates the NADase activity of prokaryotic SPARTA immune system." Nat.Chem.Biol., 20, 503-511. doi: 10.1038/s41589-023-01479-z.
- Abstract
- Argonaute proteins (Agos), which use small RNAs or DNAs as guides to recognize complementary nucleic acid targets, mediate RNA silencing in eukaryotes. In prokaryotes, Agos are involved in immunity: the short prokaryotic Ago/TIR-APAZ (SPARTA) immune system triggers cell death by degrading NAD+ in response to invading plasmids, but its molecular mechanisms remain unknown. Here we used cryo-electron microscopy to determine the structures of inactive monomeric and active tetrameric Crenotalea thermophila SPARTA complexes, revealing mechanisms underlying SPARTA assembly, RNA-guided recognition of target single-stranded DNA (ssDNA) and subsequent SPARTA tetramerization, as well as tetramerization-dependent NADase activation. The small RNA guides Ago to recognize its ssDNA target, inducing SPARTA tetramerization via both Ago- and TIR-mediated interactions and resulting in a two-stranded, parallel, head-to-tail TIR rearrangement primed for NAD+ hydrolysis. Our findings thus identify the molecular basis for target ssDNA-mediated SPARTA activation, which will facilitate the development of SPARTA-based biotechnological tools.