DSSR-PyMOL cartoon-block schematics: PDB entry 8h2h

PDB-id

8h2h; DSSR-derived features in text and JSON formats

Class

RNA binding protein-RNA

Method

cryo-EM (3.2 Å)

Summary

cryo-EM structure of a group ii intron complexed with its reverse transcriptase

Reference

Lu Y, Liu N, Liu Y, Zheng L, Yang J, Wang J, Jia X, Zi Q, Peng H, Rao Y, Wang HW (2022): "Functionalized graphene grids with various charges for single-particle cryo-EM." Nat Commun, 13, 6718. doi: 10.1038/s41467-022-34579-w.

Abstract

A major hurdle for single particle cryo-EM in structural determination lies in the specimen preparation impaired by the air-water interface (AWI) and preferential particle-orientation problems. In this work, we develop functionalized graphene grids with various charges via a dediazoniation reaction for cryo-EM specimen preparation. The graphene grids are paraffin-assistant fabricated, which appear with less contaminations compared with those produced by polymer transfer method. By applying onto three different types of macromolecules, we demonstrate that the high-yield charged graphene grids bring macromolecules away from the AWI and enable adjustable particle-orientation distribution for more robust single particle cryo-EM structural determination.