Summary information and primary citation
- PDB-id
-
7vtn;
DSSR-derived features in text and
JSON formats
- Class
- RNA binding protein-RNA
- Method
- cryo-EM (3.38 Å)
- Summary
- cryo-EM structure of the cas13bt3-crrna-target RNA
ternary complex
- Reference
-
Nakagawa R, Kannan S, Altae-Tran H, Takeda SN, Tomita A,
Hirano H, Kusakizako T, Nishizawa T, Yamashita K, Zhang
F, Nishimasu H, Nureki O (2022): "Structure
and engineering of the minimal type VI
CRISPR-Cas13bt3." Mol.Cell,
82, 3178-3192.e5. doi: 10.1016/j.molcel.2022.08.001.
- Abstract
- Type VI CRISPR-Cas13 effector enzymes catalyze
RNA-guided RNA cleavage and have been harnessed for various
technologies, such as RNA detection, targeting, and
editing. Recent studies identified Cas13bt3 (also known as
Cas13X.1) as a miniature Cas13 enzyme, which can be used
for knockdown and editing of target transcripts in
mammalian cells. However, the action mechanism of the
compact Cas13bt3 remains unknown. Here, we report the
structures of the Cas13bt3-guide RNA complex and the
Cas13bt3-guide RNA-target RNA complex. The structures
revealed how Cas13bt3 recognizes the guide RNA and its
target RNA and provided insights into the activation
mechanism of Cas13bt3, which is distinct from those of the
other Cas13a/d enzymes. Furthermore, we rationally
engineered enhanced Cas13bt3 variants and ultracompact RNA
base editors. Overall, this study improves our mechanistic
understanding of the CRISPR-Cas13 enzymes and paves the way
for the development of efficient Cas13-mediated
transcriptome modulation technologies.
