Summary information and primary citation
- PDB-id
-
7qiy;
SNAP-derived features in text and
JSON formats
- Class
- ribosome
- Method
- cryo-EM (2.58 Å)
- Summary
- Specific features and methylation sites of a plant
ribosome. 40s head ribosomal subunit.
- Reference
-
Cottilli P, Itoh Y, Nobe Y, Petrov AS, Lison P, Taoka M,
Amunts A (2022): "Cryo-EM
structure and rRNA modification sites of a plant
ribosome." Plant Commun.,
3, 100342. doi: 10.1016/j.xplc.2022.100342.
- Abstract
- Protein synthesis in crop plants contributes to the
balance of food and fuel on our planet, which influences
human metabolic activity and lifespan. Protein synthesis
can be regulated with respect to changing environmental
cues via the deposition of chemical modifications into
rRNA. Here, we present the structure of a plant ribosome
from tomato and a quantitative mass spectrometry analysis
of its rRNAs. The study reveals fine features of the
ribosomal proteins and 71 plant-specific rRNA
modifications, and it re-annotates 30 rRNA residues in the
available sequence. At the protein level, isoAsp is found
in position 137 of uS11, and a zinc finger previously
believed to be universal is missing from eL34, suggesting a
lower effect of zinc deficiency on protein synthesis in
plants. At the rRNA level, the plant ribosome differs
markedly from its human counterpart with respect to the
spatial distribution of modifications. Thus, it represents
an additional layer of gene expression regulation,
highlighting the molecular signature of a plant ribosome.
The results provide a reference model of a plant ribosome
for structural studies and an accurate marker for molecular
ecology.
