DSSR-PyMOL cartoon-block schematics: PDB entry 7qb3

PDB-id

7qb3; DSSR-derived features in text and JSON formats

Class

DNA

Method

NMR

Summary

Solution structure of a lanthanide-binding DNA aptamer

Reference

Andralojc W, Wieruszewska J, Pasternak K, Gdaniec Z (2022): "Solution Structure of a Lanthanide-binding DNA Aptamer Determined Using High Quality pseudocontact shift restraints." Chemistry, 28, e202202114. doi: 10.1002/chem.202202114.

Abstract

In this contribution we report the high-resolution NMR structure of a recently identified lanthanide-binding aptamer (LnA). We demonstrate that the rigid lanthanide binding by LnA allows for the measurement of anisotropic paramagnetic NMR restraints which to date remain largely inaccessible for nucleic acids. One type of such restraints - pseudocontact shifts (PCS) induced by four different paramagnetic lanthanides - was extensively used throughout the current structure determination study and the measured PCS turned out to be exceptionally well reproduced by the final aptamer structure. This finding opens the perspective for a broader application of paramagnetic effects in NMR studies of nucleic acids through the transplantation of the binding site found in LnA into other DNA/RNA systems.