Summary information and primary citation
- PDB-id
- 6ol3; DSSR-derived features in text and JSON formats
- Class
- RNA
- Method
- X-ray (2.74 Å)
- Summary
- Crystal structure of an adenovirus virus-associated RNA
- Reference
- Hood IV, Gordon JM, Bou-Nader C, Henderson FE, Bahmanjah S, Zhang J (2019): "Crystal structure of an adenovirus virus-associated RNA." Nat Commun, 10, 2871. doi: 10.1038/s41467-019-10752-6.
- Abstract
- Adenovirus Virus-Associated (VA) RNAs are the first discovered viral noncoding RNAs. By mimicking double-stranded RNAs (dsRNAs), the exceptionally abundant, multifunctional VA RNAs sabotage host machineries that sense, transport, process, or edit dsRNAs. How VA-I suppresses PKR activation despite its strong dsRNA character, and inhibits the crucial antiviral kinase to promote viral translation, remains largely unknown. Here, we report a 2.7 Å crystal structure of VA-I RNA. The acutely bent VA-I features an unusually structured apical loop, a wobble-enriched, coaxially stacked apical and tetra-stems necessary and sufficient for PKR inhibition, and a central domain pseudoknot that resembles codon-anticodon interactions and prevents PKR activation by VA-I. These global and local structural features collectively define VA-I as an archetypal PKR inhibitor made of RNA. The study provides molecular insights into how viruses circumnavigate cellular rules of self vs non-self RNAs to not only escape, but further compromise host innate immunity.
