DSSR-PyMOL cartoon-block schematics: PDB entry 6nut

PDB-id

6nut; DSSR-derived features in text and JSON formats

Class

viral protein-RNA

Method

cryo-EM (3.1 Å)

Summary

Ebola virus nucleoprotein - RNA complex

Reference

Kirchdoerfer RN, Saphire EO, Ward AB (2019): "Cryo-EM structure of the Ebola virus nucleoprotein-RNA complex." Acta Crystallogr.,Sect.F, 75, 340-347. doi: 10.1107/S2053230X19004424.

Abstract

Ebola virus is an emerging virus that is capable of causing a deadly disease in humans. Replication, transcription and packaging of the viral genome are carried out by the viral nucleocapsid. The nucleocapsid is a complex of the viral nucleoprotein, RNA and several other viral proteins. The nucleoprotein forms large, RNA-bound, helical filaments and acts as a scaffold for additional viral proteins. The 3.1 Å resolution single-particle cryo-electron microscopy structure of the nucleoprotein-RNA helical filament presented here resembles previous structures determined at lower resolution, while providing improved molecular details of protein-protein and protein-RNA interactions. The higher resolution of the structure presented here will facilitate the design and characterization of novel and specific Ebola virus therapeutics targeting the nucleocapsid.