Summary information and primary citation

PDB-id
6noa; DSSR-derived features in text and JSON formats
Class
RNA
Method
NMR
Summary
Solution structure of the RNA element that recruits eif3 to the 5'-utr of c-jun and regulates specialized translation initiation (apical loop)
Reference
Walker MJ, Shortridge MD, Albin DD, Cominsky LY, Varani G (2020): "Structure of the RNA Specialized Translation Initiation Element that Recruits eIF3 to the 5'-UTR of c-Jun." J.Mol.Biol., 432, 1841-1855. doi: 10.1016/j.jmb.2020.01.001.
Abstract
Specialized translation initiation is a novel form of regulation of protein synthesis, whereby RNA structures within the 5'-UTR regulate translation rates of specific mRNAs. Similar to internal ribosome entry sites (IRESs), specialized translation initiation requires the recruitment of eukaryotic initiation factor 3 (eIF3), but also requires cap recognition by eIF3d, a new 5'-m7GTP recognizing protein. How these RNA structures mediate eIF3 recruitment to affect translation of specific mRNAs remains unclear. Here, we report the nuclear magnetic resonance (NMR) structure of a stem-loop within the c-JUN 5' UTR recognized by eIF3 and essential for specialized translation initiation of this well-known oncogene. The structure exhibits similarity to eIF3 recognizing motifs found in hepatitis C virus (HCV)-like IRESs, suggesting mechanistic similarities. This work establishes the RNA structural features involved in c-JUN specialized translation initiation and provides a basis to search for small molecule inhibitors of aberrant expression of the proto-oncogenic c-JUN.

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