Summary information and primary citation
- PDB-id
- 6ido; DSSR-derived features in text and JSON formats
- Class
- transcription
- Method
- X-ray (3.748 Å)
- Summary
- Crystal structure of klebsiella pneumoniae sigma4 of sigmas fusing with the RNA polymerase beta-flap-tip-helix in complex with -35 element DNA
- Reference
- Lou YC, Chou CC, Yeh HH, Chien CY, Sadotra S, Hsu CH, Chen C (2020): "Structural basis for -35 element recognition by sigma4chimera proteins and their interactions with PmrA response regulator." Proteins, 88, 69-81. doi: 10.1002/prot.25768.
- Abstract
- In class II transcription activation, the transcription factor normally binds to the promoter near the -35 position and contacts the domain 4 of σ factors (σ4 ) to activate transcription. However, σ4 of σ70 appears to be poorly folded on its own. Here, by fusing σ4 with the RNA polymerase β-flap-tip-helix, we constructed two σ4 chimera proteins, one from σ70