Summary information and primary citation
- PDB-id
-
5gxh;
DSSR-derived features in text and
JSON formats
- Class
- RNA binding protein-RNA
- Method
- X-ray (1.8 Å)
- Summary
- The structure of the gemin5 wd40 domain with
aauuuuug
- Reference
-
Xu C, Ishikawa H, Izumikawa K, Li L, He H, Nobe Y,
Yamauchi Y, Shahjee HM, Wu X-H, Yu Y-T, Isobe T,
Takahashi N, Min J (2016): "Structural
insights into Gemin5-guided selection of pre-snRNAs for
snRNP assembly." Genes Dev.,
30, 2376-2390. doi: 10.1101/gad.288340.116.
- Abstract
- In cytoplasm, the survival of motor neuron (SMN)
complex delivers pre-small nuclear RNAs (pre-snRNAs) to the
heptameric Sm ring for the assembly of the ring complex on
pre-snRNAs at the conserved Sm site
[A(U)<sub>4-6</sub>G]. Gemin5, a WD40 protein
component of the SMN complex, is responsible for
recognizing pre-snRNAs. In addition, Gemin5 has been
reported to specifically bind to the
m<sub>7</sub>G cap. In this study, we show that
the WD40 domain of Gemin5 is both necessary and sufficient
for binding the Sm site of pre-snRNAs by isothermal
titration calorimetry (ITC) and mutagenesis assays. We
further determined the crystal structures of the WD40
domain of Gemin5 in complex with the Sm site or
m<sub>7</sub>G cap of pre-snRNA, which reveal
that the WD40 domain of Gemin5 recognizes the Sm site and
m<sub>7</sub>G cap of pre-snRNAs via two
distinct binding sites by respective base-specific
interactions. In addition, we also uncovered a novel role
of Gemin5 in escorting the truncated forms of U1 pre-snRNAs
for proper disposal. Overall, the elucidated Gemin5
structures will contribute to a better understanding of
Gemin5 in small nuclear ribonucleic protein (snRNP)
biogenesis as well as, potentially, other cellular
activities.
