Summary information and primary citation
- PDB-id
-
5fn1;
SNAP-derived features in text and
JSON formats
- Class
- virus
- Method
- cryo-EM (3.9 Å)
- Summary
- Electron cryo-microscopy of filamentous flexible virus
pepmv (pepino mosaic virus)
- Reference
-
Agirrezabala X, Mendez-Lopez E, Lasso G, Sanchez-Pina MA,
Aranda M, Valle M (2015): "The
near-atomic cryoEM structure of a flexible filamentous
plant virus shows homology of its coat protein with
nucleoproteins of animal viruses." Elife,
4, e11795. doi: 10.7554/eLife.11795.
- Abstract
- Flexible filamentous viruses include economically
important plant pathogens. Their viral particles contain
several hundred copies of a helically arrayed coat protein
(CP) protecting a (+)ssRNA. We describe here a structure at
3.9 Å resolution, from electron cryomicroscopy, of Pepino
mosaic virus (PepMV), a representative of the genus
Potexvirus (family Alphaflexiviridae). Our results allow
modeling of the CP and its interactions with viral RNA. The
overall fold of PepMV CP resembles that of nucleoproteins
(NPs) from the genus Phlebovirus (family Bunyaviridae), a
group of enveloped (-)ssRNA viruses. The main difference
between potexvirus CP and phlebovirus NP is in their
C-terminal extensions, which appear to determine the
characteristics of the distinct multimeric assemblies - a
flexuous, helical rod or a loose ribonucleoprotein. The
homology suggests gene transfer between eukaryotic (+) and
(-)ssRNA viruses.
