Summary information and primary citation
- PDB-id
-
5b1m;
DSSR-derived features in text and
JSON formats
- Class
- structural protein-DNA
- Method
- X-ray (2.34 Å)
- Summary
- The mouse nucleosome structure containing h3.1
- Reference
-
Ueda J, Harada A, Urahama T, Machida S, Maehara K, Hada
M, Makino Y, Nogami J, Horikoshi N, Osakabe A, Taguchi H,
Tanaka H, Tachiwana H, Yao T, Yamada M, Iwamoto T,
Isotani A, Ikawa M, Tachibana T, Okada Y, Kimura H,
Ohkawa Y, Kurumizaka H, Yamagata K (2017): "Testis-Specific
Histone Variant H3t Gene Is Essential for Entry into
Spermatogenesis." Cell Rep,
18, 593-600. doi: 10.1016/j.celrep.2016.12.065.
- Abstract
- Cellular differentiation is associated with dynamic
chromatin remodeling in establishing a cell-type-specific
epigenomic landscape. Here, we find that mouse
testis-specific and replication-dependent histone H3
variant H3t is essential for very early stages
of spermatogenesis. H3t gene deficiency leads to
azoospermia because of the loss of haploid germ cells. When
differentiating spermatogonia emerge in normal
spermatogenesis, H3t appears and replaces the canonical H3
proteins. Structural and biochemical analyses reveal that
H3t-containing nucleosomes are more flexible than the
canonical nucleosomes. Thus, by incorporating H3t into the
genome during spermatogonial differentiation, male germ
cells are able to enter meiosis and beyond.
