Summary information and primary citation

5ay3; DSSR-derived features in text and JSON formats
X-ray (1.2 Å)
Crystal structure of RNA duplex containing c-c base pairs
Kondo J, Tada Y, Dairaku T, Saneyoshi H, Okamoto I, Tanaka Y, Ono A (2015): "High-Resolution Crystal Structure of a Silver(I)-RNA Hybrid Duplex Containing Watson-Crick-like CSilver(I)C Metallo-Base Pairs." Angew.Chem.Int.Ed.Engl., 54, 13323-13326. doi: 10.1002/anie.201507894.
Metallo-base pairs have been extensively studied for applications in nucleic acid-based nanodevices and genetic code expansion. Metallo-base pairs composed of natural nucleobases are attractive because nanodevices containing natural metallo-base pairs can be easily prepared from commercially available sources. Previously, we have reported a crystal structure of a DNA duplex containing THg(II) T base pairs. Herein, we have determined a high-resolution crystal structure of the second natural metallo-base pair between pyrimidine bases CAg(I) C formed in an RNA duplex. One Ag(I) occupies the center between two cytosines and forms a CAg(I) C base pair through N3Ag(I) N3 linear coordination. The CAg(I) C base pair formation does not disturb the standard A-form conformation of RNA. Since the CAg(I) C base pair is structurally similar to the canonical Watson-Crick base pairs, it can be a useful building block for structure-based design and fabrication of nucleic acid-based nanodevices.

Cartoon-block schematics in six views (download the tarball)

PyMOL session file Download PDB file View in 3Dmol.js