Summary information and primary citation
- PDB-id
-
4zt0;
DSSR-derived features in text and
JSON formats
- Class
- hydrolase-RNA
- Method
- X-ray (2.9 Å)
- Summary
- Crystal structure of catalytically-active streptococcus
pyogenes cas9 in complex with single-guide RNA at 2.9
angstrom resolution
- Reference
-
Jiang F, Zhou K, Ma L, Gressel S, Doudna JA (2015):
"A
Cas9-guide RNA complex preorganized for target DNA
recognition." Science, 348,
1477-1481. doi: 10.1126/science.aab1452.
- Abstract
- Bacterial adaptive immunity uses CRISPR (clustered
regularly interspaced short palindromic repeats)-associated
(Cas) proteins together with CRISPR transcripts for foreign
DNA degradation. In type II CRISPR-Cas systems, activation
of Cas9 endonuclease for DNA recognition upon guide RNA
binding occurs by an unknown mechanism. Crystal structures
of Cas9 bound to single-guide RNA reveal a conformation
distinct from both the apo and DNA-bound states, in which
the 10-nucleotide RNA "seed" sequence required for initial
DNA interrogation is preordered in an A-form conformation.
This segment of the guide RNA is essential for Cas9 to form
a DNA recognition-competent structure that is poised to
engage double-stranded DNA target sequences. We construe
this as convergent evolution of a "seed" mechanism
reminiscent of that used by Argonaute proteins during RNA
interference in eukaryotes.
