Summary information and primary citation
- PDB-id
-
3trz;
DSSR-derived features in text and
JSON formats
- Class
- RNA binding protein-RNA
- Method
- X-ray (2.9 Å)
- Summary
- Mouse lin28a in complex with let-7d microrna
pre-element
- Reference
-
Nam Y, Chen C, Gregory RI, Chou JJ, Sliz P (2011):
"Molecular
Basis for Interaction of let-7 MicroRNAs with Lin28."
Cell(Cambridge,Mass.), 147,
1080-1091. doi: 10.1016/j.cell.2011.10.020.
- Abstract
- MicroRNAs (miRNAs) are small noncoding RNA molecules
that regulate gene expression. Among these, members of the
let-7 miRNA family control many cell-fate determination
genes to influence pluripotency, differentiation, and
transformation. Lin28 is a specific, posttranscriptional
inhibitor of let-7 biogenesis. We report crystal structures
of mouse Lin28 in complex with sequences from let-7d,
let-7-f1, and let-7 g precursors. The two folded domains of
Lin28 recognize two distinct regions of the RNA and are
sufficient for inhibition of let-7 in vivo. We also show by
NMR spectroscopy that the linker connecting the two folded
domains is flexible, accommodating Lin28 binding to diverse
let-7 family members. Protein-RNA complex formation imposes
specific conformations on both components that could affect
downstream recognition by other processing factors. Our
data provide a molecular explanation for Lin28 specificity
and a model for how it regulates let-7.
