DSSR-PyMOL cartoon-block schematics: PDB entry 3sqi

PDB-id

3sqi; DSSR-derived features in text and JSON formats

Class

DNA binding protein-DNA

Method

X-ray (2.82 Å)

Summary

DNA binding domain of ndc10

Reference

Cho US, Harrison SC (2011): "Ndc10 is a platform for inner kinetochore assembly in budding yeast." Nat.Struct.Mol.Biol., 19, 48-55. doi: 10.1038/nsmb.2178.

Abstract

Kinetochores link centromeric DNA to spindle microtubules and ensure faithful chromosome segregation during mitosis. In point-centromere yeasts, the CBF3 complex Skp1-Ctf13-(Cep3)(2)-(Ndc10)(2) recognizes a conserved centromeric DNA element through contacts made by Cep3 and Ndc10. We describe here the five-domain organization of Kluyveromyces lactis Ndc10 and the structure at 2.8 Å resolution of domains I-II (residues 1-402) bound to DNA. The structure resembles tyrosine DNA recombinases, although it lacks both endonuclease and ligase activities. Structural and biochemical data demonstrate that each subunit of the Ndc10 dimer binds a separate fragment of DNA, suggesting that Ndc10 stabilizes a DNA loop at the centromere. We describe in vitro association experiments showing that specific domains of Ndc10 interact with each of the known inner-kinetochore proteins or protein complexes in budding yeast. We propose that Ndc10 provides a central platform for inner-kinetochore assembly.