Summary information and primary citation
- PDB-id
-
3skr;
SNAP-derived features in text and
JSON formats
- Class
- RNA
- Method
- X-ray (3.1 Å)
- Summary
- Crystal structure of the 2'- deoxyguanosine riboswitch
bound to 2'- deoxyguanosine, cobalt hexammine soak
- Reference
-
Pikovskaya O, Polonskaia A, Patel DJ, Serganov A (2011):
"Structural
principles of nucleoside selectivity in a
2'-deoxyguanosine riboswitch."
Nat.Chem.Biol., 7, 748-755.
doi: 10.1038/nchembio.631.
- Abstract
- Purine riboswitches have an essential role in genetic
regulation of bacterial metabolism. This family includes
the 2'-deoxyguanosine (dG) riboswitch, which is involved in
feedback control of deoxyguanosine biosynthesis. To
understand the principles that define dG selectivity, we
determined crystal structures of the natural Mesoplasma
florum riboswitch bound to cognate dG as well as to
noncognate guanosine, deoxyguanosine monophosphate and
guanosine monophosphate. Comparison with related purine
riboswitch structures reveals that the dG riboswitch
achieves its specificity through modification of key
interactions involving the nucleobase and rearrangement of
the ligand-binding pocket to accommodate the additional
sugar moiety. In addition, we observe new conformational
changes beyond the junctional binding pocket extending as
far as peripheral loop-loop interactions. It appears that
re-engineering riboswitch scaffolds will require
consideration of selectivity features dispersed throughout
the riboswitch tertiary fold, and structure-guided drug
design efforts targeted to junctional RNA scaffolds need to
be addressed within such an expanded framework.
