Summary information and primary citation
- PDB-id
-
3qrr;
DSSR-derived features in text and
JSON formats
- Class
- RNA binding protein-RNA
- Method
- X-ray (3.099 Å)
- Summary
- Structure of thermus thermophilus cse3 bound to an RNA
representing a product complex
- Reference
-
Gesner EM, Schellenberg MJ, Garside EL, George MM,
Macmillan AM (2011): "Recognition
and maturation of effector RNAs in a CRISPR interference
pathway." Nat.Struct.Mol.Biol.,
18, 688-692. doi: 10.1038/nsmb.2042.
- Abstract
- In bacteria and archaea, small RNAs derived from
clustered, regularly interspaced, short palindromic repeat
(CRISPR) loci are involved in an adaptable and heritable
gene-silencing pathway. Resistance to phage infection is
conferred by the incorporation of short invading DNA
sequences into the genome as CRISPR spacer elements
separated by short repeat sequences. Processing of long
primary transcripts (pre-crRNAs) containing these repeats
by an RNA endonuclease generates the mature effector RNAs
that interfere with phage gene expression. Here we describe
structural and functional analyses of the Thermus
thermophilus CRISPR Cse3 endonuclease. High-resolution
X-ray structures of Cse3 bound to repeat RNAs model both
the pre- and post-cleavage complexes associated with
processing the pre-crRNA. These structures establish the
molecular basis of a specific CRISPR RNA recognition and
suggest the mechanism for generation of effector RNAs
responsible for gene silencing.
