Summary information and primary citation

PDB-id
3izd; DSSR-derived features in text and JSON formats
Class
ribosome
Method
cryo-EM (8.6 Å)
Summary
Model of the large subunit RNA expansion segment es27l-out based on a 6.1 a cryo-EM map of saccharomyces cerevisiae translating 80s ribosome. 3izd is a small part (an expansion segment) which is in an alternative conformation to what is in already 3izf.
Reference
Armache JP, Jarasch A, Anger AM, Villa E, Becker T, Bhushan S, Jossinet F, Habeck M, Dindar G, Franckenberg S, Marquez V, Mielke T, Thomm M, Berninghausen O, Beatrix B, Soding J, Westhof E, Wilson DN, Beckmann R (2010): "Cryo-EM structure and rRNA model of a translating eukaryotic 80S ribosome at 5.5-A resolution." Proc.Natl.Acad.Sci.USA, 107, 19748-19753. doi: 10.1073/pnas.1009999107.
Abstract
Protein biosynthesis, the translation of the genetic code into polypeptides, occurs on ribonucleoprotein particles called ribosomes. Although X-ray structures of bacterial ribosomes are available, high-resolution structures of eukaryotic 80S ribosomes are lacking. Using cryoelectron microscopy and single-particle reconstruction, we have determined the structure of a translating plant (Triticum aestivum) 80S ribosome at 5.5-Å resolution. This map, together with a 6.1-Å map of a Saccharomyces cerevisiae 80S ribosome, has enabled us to model ∼98% of the rRNA. Accurate assignment of the rRNA expansion segments (ES) and variable regions has revealed unique ES-ES and r-protein-ES interactions, providing insight into the structure and evolution of the eukaryotic ribosome.

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