Summary information and primary citation
- PDB-id
- 3i4m; DSSR-derived features in text and JSON formats
- Class
- transcription,transferase-DNA-RNA hybrid
- Method
- X-ray (3.7 Å)
- Summary
- 8-oxoguanine containing RNA polymerase ii elongation complex d
- Reference
- Damsma GE, Cramer P (2009): "Molecular basis of transcriptional mutagenesis at 8-oxoguanine." J.Biol.Chem., 284, 31658-31663. doi: 10.1074/jbc.M109.022764.
- Abstract
- Structure-function analysis has revealed the mechanism of yeast RNA polymerase II transcription at 8-oxoguanine (8-oxoG), the major DNA lesion resulting from oxidative stress. When polymerase II encounters 8-oxoG in the DNA template strand, it can misincorporate adenine, which forms a Hoogsteen bp with 8-oxoG at the active center. This requires rotation of the 8-oxoG base from the standard anti- to an uncommon syn-conformation, which likely occurs during 8-oxoG loading into the active site. The misincorporated adenine escapes intrinsic proofreading, resulting in transcriptional mutagenesis that is observed directly by mass spectrometric RNA analysis.
