Summary information and primary citation

PDB-id
3bnt; DSSR-derived features in text and JSON formats
Class
RNA
Method
X-ray (2.3 Å)
Summary
Crystal structure of the homo sapiens mitochondrial ribosomal decoding site in the presence of [co(nh3)6]cl3 (a1555g mutant, br-derivative)
Reference
Kondo J, Westhof E (2008): "The bacterial and mitochondrial ribosomal A-site molecular switches possess different conformational substates." Nucleic Acids Res., 36, 2654-2666. doi: 10.1093/nar/gkn112.
Abstract
The A site of the small ribosomal subunit participates in the fidelity of decoding by switching between two states, a resting 'off' state and an active decoding 'on' state. Eight crystal structures of RNA duplexes containing two minimal decoding A sites of the Homo sapiens mitochondrial wild-type, the A1555G mutant or bacteria have been solved. The resting 'off' state of the mitochondrial wild-type A site is surprisingly different from that of the bacterial A site. The mitochondrial A1555G mutant has two types of the 'off' states; one is similar to the mitochondrial wild-type 'off' state and the other is similar to the bacterial 'off' state. Our present results indicate that the dynamics of the A site in bacteria and mitochondria are different, a property probably related to the small number of tRNAs used for decoding in mitochondria. Based on these structures, we propose a hypothesis for the molecular mechanism of non-syndromic hearing loss due to the mitochondrial A1555G mutation.

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