Summary information and primary citation
- PDB-id
- 353d; DSSR-derived features in text and JSON formats
- Class
- RNA
- Method
- X-ray (2.4 Å)
- Summary
- Crystal structure of domain a of thermus flavus 5s rrna and the contribution of water molecules to its structure
- Reference
- Betzel C, Lorenz S, Furste JP, Bald R, Zhang M, Schneider TR, Wilson KS, Erdmann VA (1994): "Crystal structure of domain A of Thermus flavus 5S rRNA and the contribution of water molecules to its structure." FEBS Lett., 351, 159-164. doi: 10.1016/0014-5793(94)00834-5.
- Abstract
- This is the first high resolution crystal structure of an RNA molecule made by solid phase chemical synthesis and representing a natural RNA. The structure of the domain A of Thermus flavus ribosomal 5S RNA is refined to R = 18% at 2.4 A including 159 solvent molecules. Most of the 2'-hydroxyl groups as well as the phosphate oxygens are involved either in specific hydrogen bonds in intermolecular contacts or to solvent molecules. The two U-G and G-U base-pairs are stabilized by H-bonds supplied via three water molecules to compensate for the lack of base-pair hydrogen bonds. The structure shows for the first time in detail the importance of highly ordered internal water in stabilizing an RNA structure.
