Summary information and primary citation
- PDB-id
-
2y9z;
DSSR-derived features in text and
JSON formats
- Class
- transcription
- Method
- X-ray (3.601 Å)
- Summary
- Chromatin remodeling factor isw1a(del_atpase) in DNA
complex
- Reference
-
Yamada K, Frouws TD, Angst B, Fitzgerald DJ, Deluca C,
Schimmele K, Sargent DF, Richmond TJ (2011): "Structure
and Mechanism of the Chromatin Remodelling Factor
Isw1A." Nature, 472, 448.
doi: 10.1038/NATURE09947.
- Abstract
- Site-specific recognition of DNA in eukaryotic
organisms depends on the arrangement of nucleosomes in
chromatin. In the yeast Saccharomyces cerevisiae, ISW1a and
related chromatin remodelling factors are implicated in
establishing the nucleosome repeat during replication and
altering nucleosome position to affect gene activity. Here
we have solved the crystal structures of S. cerevisiae
ISW1a lacking its ATPase domain both alone and with DNA
bound at resolutions of 3.25 Å and 3.60 Å, respectively,
and we have visualized two different nucleosome-containing
remodelling complexes using cryo-electron microscopy. The
composite X-ray and electron microscopy structures combined
with site-directed photocrosslinking analyses of these
complexes suggest that ISW1a uses a dinucleosome substrate
for chromatin remodelling. Results from a remodelling assay
corroborate the dinucleosome model. We show how a chromatin
remodelling factor could set the spacing between two
adjacent nucleosomes acting as a 'protein ruler'.
