DSSR-PyMOL cartoon-block schematics: PDB entry 2qh2

PDB-id

2qh2; DSSR-derived features in text and JSON formats

Class

RNA

Method

NMR

Summary

Solution structure of the cr7 terminal hairpin loop from human telomerase RNA

Reference

Theimer CA, Jady BE, Chim N, Richard P, Breece KE, Kiss T, Feigon J (2007): "Structural and functional characterization of human telomerase RNA processing and cajal body localization signals." Mol.Cell, 27, 869-881. doi: 10.1016/j.molcel.2007.07.017.

Abstract

The RNA component of human telomerase (hTR) includes H/ACA and CR7 domains required for 3' end processing, localization, and accumulation. The terminal loop of the CR7 domain contains the CAB box (ugAG) required for targeting of scaRNAs to Cajal bodies (CB) and an uncharacterized sequence required for accumulation and processing. To dissect out the contributions of the CR7 stem loop to hTR processing and localization, we solved the solution structures of the 3' terminal stem loops of hTR CR7 and U64 H/ACA snoRNA, and the 5' terminal stem loop of U85 C/D-H/ACA scaRNA. These structures, together with analysis of localization, processing, and accumulation of hTRs containing nucleotide substitutions in the CR7 domain, identified the sequence and structural requirements of the hTR processing and CB localization signals and showed that these signals are functionally independent. Further, 3' end processing was found to be a prerequisite for translocation of hTR to CBs.