Summary information and primary citation
- PDB-id
-
2o8k;
SNAP-derived features in text and
JSON formats
- Class
- transcription-DNA
- Method
- NMR
- Summary
- NMR structure of the sigma-54 rpon domain bound to
the-24 promoter element
- Reference
-
Doucleff M, Pelton JG, Lee PS, Nixon BT, Wemmer DE
(2007): "Structural
basis of DNA recognition by the alternative sigma-factor,
sigma54." J.Mol.Biol., 369,
1070-1078. doi: 10.1016/j.jmb.2007.04.019.
- Abstract
- The sigma subunit of bacterial RNA polymerase (RNAP)
regulates gene expression by directing RNAP to specific
promoters. Unlike sigma(70)-type proteins, the alternative
sigma factor, sigma(54), requires interaction with an
ATPase to open DNA. We present the solution structure of
the C-terminal domain of sigma(54) bound to the -24
promoter element, in which the conserved RpoN box motif
inserts into the major groove of the DNA. This structure
elucidates the basis for sequence specific recognition of
the -24 element, orients sigma(54) on the promoter, and
suggests how the C-terminal domain of sigma(54) interacts
with RNAP.
