Summary information and primary citation
- PDB-id
-
2mcj;
DSSR-derived features in text and
JSON formats
- Class
- DNA
- Method
- NMR
- Summary
- NMR structure of spermine modified DNA duplex
- Reference
-
Brzezinska J, Gdaniec Z, Popenda L, Markiewicz WT (2014):
"Polyaminooligonucleotide:
NMR structure of duplex DNA containing a nucleoside with
spermine residue,
N-[4,9,13-triazatridecan-1-yl]-2'-deoxycytidine."
Biochim.Biophys.Acta, 1840,
1163-1170. doi: 10.1016/j.bbagen.2013.12.008.
- Abstract
- Background: The nature of the polyamine-DNA
interactions at a molecular level is not clearly
understood.
Methods: In order to shed light on the binding preferences
of polyamine with nucleic acids, the NMR solution structure
of the DNA duplex containing covalently bound spermine was
determined.
Results: The structure of
4-N-[4,9,13-triazatridecan-1-yl]-2'-deoxycytidine (dCSp)
modified duplex was compared to the structure of the
reference duplex. Both duplexes are regular right-handed
helices with all attributes of the B-DNA form. The spermine
chain which is located in a major groove and points toward
the 3' end of the modified strand does not perturb the DNA
structure.
