Summary information and primary citation
- PDB-id
- 2mcj; DSSR-derived features in text and JSON formats
- Class
- DNA
- Method
- NMR
- Summary
- NMR structure of spermine modified DNA duplex
- Reference
- Brzezinska J, Gdaniec Z, Popenda L, Markiewicz WT (2014): "Polyaminooligonucleotide: NMR structure of duplex DNA containing a nucleoside with spermine residue, N-[4,9,13-triazatridecan-1-yl]-2'-deoxycytidine." Biochim.Biophys.Acta, 1840, 1163-1170. doi: 10.1016/j.bbagen.2013.12.008.
- Abstract
- Background: The nature of the polyamine-DNA interactions at a molecular level is not clearly understood.
Methods: In order to shed light on the binding preferences of polyamine with nucleic acids, the NMR solution structure of the DNA duplex containing covalently bound spermine was determined.
Results: The structure of 4-N-[4,9,13-triazatridecan-1-yl]-2'-deoxycytidine (dCSp) modified duplex was compared to the structure of the reference duplex. Both duplexes are regular right-handed helices with all attributes of the B-DNA form. The spermine chain which is located in a major groove and points toward the 3' end of the modified strand does not perturb the DNA structure.
