Summary information and primary citation
- 2lx1; DSSR-derived features in
text and JSON formats
- Major conformation of the internal loop 5'gagu-3'ugag
- Kennedy, S.D., Kierzek, R., Turner, D.H.: (2012) "Novel conformation of an RNA structural switch." Biochemistry, 51, 9257-9259.
- The RNA duplex, (5'GACGAGUGUCA)(2), has two conformations in equilibrium. The nuclear magnetic resonance solution structure reveals that the major conformation of the loop, 5'GAGU/3'UGAG, is novel and contains two unusual Watson-Crick/Hoogsteen GG pairs with G residues in the syn conformation, two A residues stacked on each other in the center of the helix with inverted sugars, and two bulged-out U residues. The structure provides a benchmark for testing approaches for predicting local RNA structure and a sequence that allows the design of a unique arrangement of functional groups and/or a conformational switch into nucleic acids.