Summary information and primary citation
- PDB-id
-
28sp;
DSSR-derived features in text and
JSON formats
- Class
- RNA
- Method
- NMR
- Summary
- NMR structure of the most conserved RNA motif in srp
RNA
- Reference
-
Schmitz U, James TL, Lukavsky P, Walter P (1999):
"Structure
of the most conserved internal loop in SRP RNA."
Nat.Struct.Biol., 6, 634-638.
doi: 10.1038/10683.
- Abstract
- The signal recognition particle (SRP) directs
translating ribosomes to the protein translocation
apparatus of endoplasmic reticulum (ER) membrane or the
bacterial plasma membrane. The SRP is universally
conserved, and in prokaryotes consists of two essential
subunits, SRP RNA and SRP54, the latter of which binds to
signal sequences on the nascent protein chains. Here we
describe the solution NMR structure of a 28-mer RNA
composing the most conserved part of SRP RNA to which SRP54
binds. Central to this function is a six-nucleotide
internal loop that assumes a novel Mg2+-dependent structure
with unusual cross-strand interactions; besides a
cross-strand A/A stack, two guanines form hydrogen bonds
with opposite-strand phosphates. The structure completely
explains the phylogenetic conservation of the loop bases,
underlining its importance for SRP54 binding and SRP
function.
