Summary information and primary citation
- PDB-id
- 1ma8; DSSR-derived features in text and JSON formats
- Class
- DNA
- Method
- X-ray (1.3 Å)
- Summary
- A-DNA decamer gcgta(ums)acgc with incorporated 2'-methylseleno-uridine
- Reference
- Teplova M, Wilds CJ, Wawrzak Z, Tereshko V, Du Q, Carrasco N, Huang Z, Egli M (2002): "Covalent incorporation of selenium into oligonucleotides for X-ray crystal structure determination via MAD: proof of principle." BIOCHIMIE, 84, 849-858. doi: 10.1016/S0300-9084(02)01440-2.
- Abstract
- Selenium was incorporated into an oligodeoxynucleotide in the form of 2'-methylseleno-uridine (U(Se)). The X-ray crystal structure of the duplex left open bracket d(GCGTA)U(Se)d(ACGC) right open bracket (2) was determined by the multiwavelength anomalous dispersion (MAD) technique and refined to a resolution of 1.3 A, demonstrating that selenium can selectively substitute oxygen in DNA and that the resulting compounds are chemically stable. Since derivatization at the 2'-alpha-position with selenium does not affect the preference of the sugar for the C3'-endo conformation, this strategy is suitable for incorporating selenium into RNA. The availability of selenium-containing nucleic acids for crystallographic phasing offers an attractive alternative to the commonly used halogenated pyrimidines.
