Summary information and primary citation

1jgo; DSSR-derived features in text and JSON formats
X-ray (5.6 Å)
The path of messenger RNA through the ribosome. this file, 1jgo, contains the 30s ribosome subunit, three trna, and mrna molecules. 50s ribosome subunit is in the file 1giy
Yusupova GZ, Yusupov MM, Cate JH, Noller HF (2001): "The path of messenger RNA through the ribosome." Cell(Cambridge,Mass.), 106, 233-241. doi: 10.1016/S0092-8674(01)00435-4.
Using X-ray crystallography, we have directly observed the path of mRNA in the 70S ribosome in Fourier difference maps at 7 A resolution. About 30 nucleotides of the mRNA are wrapped in a groove that encircles the neck of the 30S subunit. The Shine-Dalgarno helix is bound in a large cleft between the head and the back of the platform. At the interface, only about eight nucleotides (-1 to +7), centered on the junction between the A and P codons, are exposed, and bond almost exclusively to 16S rRNA. The mRNA enters the ribosome around position +13 to +15, the location of downstream pseudoknots that stimulate -1 translational frame shifting.

Cartoon-block schematics in six views (download the tarball)

PyMOL session file Download PDB file View in 3Dmol.js