Summary information and primary citation
- PDB-id
- 1jgo; DSSR-derived features in text and JSON formats
- Class
- ribosome
- Method
- X-ray (5.6 Å)
- Summary
- The path of messenger RNA through the ribosome. this file, 1jgo, contains the 30s ribosome subunit, three trna, and mrna molecules. 50s ribosome subunit is in the file 1giy
- Reference
- Yusupova GZ, Yusupov MM, Cate JH, Noller HF (2001): "The path of messenger RNA through the ribosome." Cell(Cambridge,Mass.), 106, 233-241. doi: 10.1016/S0092-8674(01)00435-4.
- Abstract
- Using X-ray crystallography, we have directly observed the path of mRNA in the 70S ribosome in Fourier difference maps at 7 A resolution. About 30 nucleotides of the mRNA are wrapped in a groove that encircles the neck of the 30S subunit. The Shine-Dalgarno helix is bound in a large cleft between the head and the back of the platform. At the interface, only about eight nucleotides (-1 to +7), centered on the junction between the A and P codons, are exposed, and bond almost exclusively to 16S rRNA. The mRNA enters the ribosome around position +13 to +15, the location of downstream pseudoknots that stimulate -1 translational frame shifting.
