Summary information and primary citation
- PDB-id
-
1fhk;
DSSR-derived features in text and
JSON formats
- Class
- RNA
- Method
- NMR
- Summary
- NMR structure of the 690 loop of 16 s rrna of e.
coli
- Reference
-
Morosyuk SV, Cunningham PR, SantaLucia Jr J (2001):
"Structure
and function of the conserved 690 hairpin in Escherichia
coli 16 S ribosomal RNA. II. NMR solution structure."
J.Mol.Biol., 307, 197-211. doi:
10.1006/jmbi.2000.4431.
- Abstract
- The solution structure of the conserved 690 hairpin
from Escherichia coli 16 S rRNA was determined by NMR
spectroscopy. The 690 loop is located at the surface of the
30 S subunit in the platform region and has been implicated
in interactions with P-site bound tRNA, E-site mRNA, S11
binding, IF3 binding, and in RNA-RNA interactions with the
790 loop of 16 S rRNA and domain IV of 23 S rRNA. The
structure reveals a novel sheared type G690.U697 base-pair
with a single hydrogen bond from the G690 amino to U697-04.
G691 and A696 also form a sheared pair and U692 forms a
U-turn with an H-bond to the A695 non-bridging phosphate
oxygen. The sheared pairs and U-turn result in the
continuous single-stranded stacking of five residues from
6693 to U697 with their Watson-Crick functional groups
exposed in the minor groove. The overall fold of the 690
hairpin is similar to the anticodon loop of tRNA. The
structure provides an explanation for chemical protection
patterns in the loop upon interaction with tRNA, the 50 S
subunit, and S11. In vivo genetic studies demonstrate the
functional importance of the motifs observed in the
solution structure of the 690 hairpin.
