Summary information and primary citation
- PDB-id
-
197d;
SNAP-derived features in text and
JSON formats
- Class
- DNA
- Method
- X-ray (2.19 Å)
- Summary
- Orthorhombic crystal structure of the a-DNA octamer
d(gtacgtac). comparison with the tetragonal structure
- Reference
-
Langlois d'Estaintot B, Dautant A, Courseille C,
Precigoux G (1993): "Orthorhombic
crystal structure of the A-DNA octamer d(GTACGTAC).
Comparison with the tetragonal structure."
Eur.J.Biochem., 213, 673-682.
doi: 10.1111/j.1432-1033.1993.tb17807.x.
- Abstract
- The X-ray crystal structure of the double-helical A-DNA
octanucleotide d(GTACGTAC) has been solved by molecular
replacement and refined to a resolution of 0.219 nm. The
final R-factor is equal to 16.1% for 1516 observed
reflections with F > 4 sigma(F). The sequence
crystallizes as an A-DNA-type double helix in the
orthorhombic space group P2(1)2(1)2, with one duplex
molecule solvated by 66 water molecules in the asymmetric
unit. Cell parameters are a = 3.860 nm, b = 5.082 nm, c =
2.174 nm. It is the first time that such a crystal form has
been observed. This orthorhombic structure has been
compared with the tetragonal structure of the same
oligonucleotide. It adopts a bent structure with an unusual
packing between symmetry-related molecules.
